Kemampuan Daya Hambat Bahan Aktif Beberapa Merek Dagang Hand sanitizer terhadap Pertumbuhan Staphylococcus aureus

Abstract

Abstrak

Hand sanitizer sebagai pembersih tangan antiseptik inovatif saat ini, sering menjadi alternatif pengganti cuci tangan dengan sabun dan air. Mencuci tangan dengan hand sanitizer merupakan salah satu cara memelihara kebersihan tangan agar terhindar dari penyakit  yang disebabkan oleh flora normal di kulit yang berpotensi patogenik seperti bakteri Staphylococcus aureus. Tujuan penelitian ini adalah menilai kemampuan daya hambat beberapa merek dagang hand sanitizer terhadap pertumbuhan Staphylococcus aureus. Penelitian dilakukan dengan metode rancangan acak lengkap pada empat macam hand sanitizer dengan tiga kali pengulangan. Data diolah secara manual dan menggunakan analisis varians (ANOVA) dilanjutkan dengan uji pos-hoc dengan Tukey HSD dan independent t test. Hasil penelitian menunjukkan bahwa bahan aktif hand sanitizer mampu mengurangi pertumbuhan Staphylococcus aureus secara signifikan. Perbedaan prosentase rerata pengurangan jumlah koloni pada waktu 30 detik berkisar antara 57,65%-72,45%, sedangkan pada waktu 1 menit berkisar 67,88%-82,65%. Hasil analisis menunjukkan perbedaan bermakna terhadap perlakuan, antar perlakuan dan waktu yang diujikan dengan nilai signifikasi p <0,05. Didapatkan hand sanitizer lebih efektif menghambat pertumbuhan pada waktu 1 menit daripada 30 detik. Hand sanitizer yang mengandung alkohol 70% dan triklosan 0,05% memiliki kemampuan daya hambat lebih baik terhadap pertumbuhan Staphylococcus aureus.

Kata kunci: bahan aktif, hand sanitizer, Staphylococcus aureus

Abstract

Hand sanitizer is one of the ways for maintaining the hand hygiene. Hand sanitizer as an innovative antiseptic product at this time, becomes an alternative hand washing with soap and water. It prevents  the disease that can be caused by normal flora that potentially pathogenic bacterium such as Staphylococcus aureus. The objective of this study was to evaluate the inhibition ability of several trademarks hand sanitizer on the growth of Staphylococcus aureus. The research method used a completely randomized design in four kinds of hand sanitizer and three repetitions. Data processed manually and using analysis of variance (ANOVA) and followed by Post-Hoc test with Tukey HSD and also Independent T-Test.The results showed that the ingredients of hand sanitizer can reduce the growth of Staphylococcus aureus significantly. The differences on the percentage reduction of colonies in 30 seconds ranged between 57,65%-72,45%, while the range in 1 minute was 67,88%-82,65%. The analysis was also showed a real difference to the experiment, the combination of the experiment and the time of the experiment with significance value of p <0.05. Based on the result, hand sanitizers can inhibit the growth of Staphylococcus aureus effectively at 1 minute instead of 30 seconds. Hand sanitizer containing alcohol 70% and triclosan 0.05% has the better ability to inhibit the growth of Staphylococcus aureus.

Keywords: ingredients, hand sanitizer, Staphylococcus aureus