Pengaruh Pemberian Mesenchymal Stem Cells Wharton’s Jelly terhadap Ekspresi Gen PPAR-γ pada Tikus Alzheimer

Authors

  • Nabila Priscilla Putri Program Studi Kedokteran, Fakultas Kedokteran, Universitas Andalas
  • Hirowati Ali Departemen Biokimia, Fakultas Andalas, Univerisitas Andalas
  • Tofrizal Tofrizal Bagian Patologi Anatomi, Fakultas Kedokteran, Universitas Andalas
  • Eryati Darwin Departemen Histologi, Fakultas Kedokteran, Universitas Andalas
  • Restu Susanti Departemen Neurologi, Fakultas Kedokteran, Universitas Andalas
  • Hasmiwati Hasmiwati Departemen Parasitologi, Fakultas Kedokteran, Universitas Andalas

DOI:

https://doi.org/10.25077/jka.v12i2.2142

Abstract

The increased production of beta-amyloid marks Alzheimer's disease. PPAR-γ shows involvement in Alzheimer's disease. PPAR-γ Gene is suspected to reduce beta-amyloid plaques and reduce inflammation and oxidative stress in Alzheimer's disease. Mesenchymal Stem Cells Wharton's Jelly (MSC-WJ) is expected to increase the PPAR-γ Gene expression. Objective:  To observed the PPAR-γ Gene expression in Alzheimer's Mice. Methods: The mice are induced using AlCl3 and were given MSC-WJ. This study was an experimental study with a post-test-only control group design on 18 samples of RNA from Alzheimer's mice that were divided into 3 groups. Negative control group (K-), positive control group (K+), and experimented group (P). The mean of PPAR-γ gene expression was obtained by comparing the PPAR-γ gene with the GAPDH gene. This study used semiquantitative methods using ImageJ. Data analysis was used with the Kruskal-Wallis test. It is significant if the value is <0,05. Results: The mean ratio of PPAR-γ gene expression that was obtained in K-, K+, and P were 0.12, 0.06, and 0.08, respectively. There were significant differences between each group, with a p-value of 0,023 (p<0,05). Conclusion: Giving MSC-WJ could increase the PPAR-γ gene expression. Further study using real-time PCR is highly needed to increase the MSC-WJ treatment in patients with Alzheimer's disease.

Keywords:  Alzheimer disease, MSC-WJ, PPAR- γ

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Published

2023-10-24 — Updated on 2025-03-20

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How to Cite

Putri, N. P., Ali, H., Tofrizal, T., Darwin, E., Susanti, R., & Hasmiwati, H. (2025). Pengaruh Pemberian Mesenchymal Stem Cells Wharton’s Jelly terhadap Ekspresi Gen PPAR-γ pada Tikus Alzheimer. Jurnal Kesehatan Andalas, 12(2), 70–75. https://doi.org/10.25077/jka.v12i2.2142 (Original work published October 24, 2023)

Issue

Vol. 12 No. 2 (2023): Online July 2023

Section

Research Articles

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Copyright (c) 2023 Nabila Priscilla Putri, Hirowati Ali, Tofrizal, Eryati Darwin, Restu Susanti, Hasmiwati

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