Efek Pengurangan Durasi Tidur terhadap Jumlah dan Hitung Jenis Leukosit pada Tikus Wistar

Authors

  • Wahyu Tri Novriansyah
  • Ellyza Nasrul
  • Rosfita Rasyid

DOI:

https://doi.org/10.25077/jka.v5i3.607

Abstract


Abstrak

Sleep Deprivation (SD) adalah hilangnya waktu tidur komplit  untuk periode tertentu ataupun durasi tidur yang lebih pendek dari waktu optimal yang dibutuhkan. Sleep deprivation mempengaruhi sistem kekebalan tubuh sebagaimana terganggunya irama sirkadian. Tujuan peneltian ini adalah menentukan efek dari sleep deprivation terhadap jumlah dan hitung jenis leukosit pada tikus wistar. Penelitian ini adalah studi eksperimental  terhadap 24 ekor tikus yang dibagi menjadi empat kelompok, terdiri dari kelompok kontrol (K0), kelompok perlakuan P1 (SD 48 jam), P2 (SD 72 jam) dan P3 (SD 96 jam).  Jumlah leukosit, eosinofil, neutrofil, limfosit dan monosit diperiksa dengan Blood Analyzer Impedant Pentra 60. Analisis data menggunakan one way Anova dengan signifikansi p<0,05. Hasil yang didapat ialah jumlah leukosit pada kelompok perlakuan P1, P2 dan P3 tidak menunjukkan perbedaan signifikan (12091±4712,3). Hitung eosinofil menunjukkan adanya peningkatan signifikan pada kelompok P1 (0,93±0,7) dan P2 (1,75±1,5) dibandingkan K0 (0,13±0,08). Pada P3 (0,32±0,35) terlihat penurunan hitung eosinofil yang signifikan dibandingkan dengan kelompok P2. Hitung neutrofil menurun pada kelompok P3 (6,5±8,1) dibandingkan dengan kelompok K0 (10,9±3,7) dan P2 (13,5±4,2). Hitung limfosit menurun pada P2 (78,1 ±7,3) dibanding K0 (86,3±3,9), dan meningkat pada P3 (87,4 ±6,5) dibanding P2. Monosit pada kelompok perlakuan P1, P2 dan P3 tidak menunjukkan perbedaan signifikan (2,0 ±0,5).

                            Kata kunci: sleep deprivation, leukosit, eosinofil, neutrofil, limfosit, monosit

 

Abstract

Sleep Deprivation (SD) is a complete loss of sleep for a certain period or a shorter sleep duration than the optimal time required. Sleep deprivation affects the immune system as well as the disruption of circadian rhythms. The objective of this study was to determine the effect of sleep deprivation on total and differential counting of leukocytes. This was an experimental study on 24 rats which divided into four groups, consisting of the control group (K0), the treatment group P1 (SD 48 hours), P2 (SD 72 hours), and P3 (SD 96 hours). After treatment, total number leukocytes, eosinophils, neutrophils, lymphocytes and monocytes were calculated using a blood analyzer impedant pentra 60. Analysis of the data using a one way ANOVA with significance p <0.05. The number of leukocytes in the treated group P1, P2 and P3 showed no significant difference (12091 ± 4712.3). Eosinophil count results showed a significant increase in the P1 group (0.93 ± 0.7) and P2 (1.75 ± 1.5) compared to K0 (0.13 ± 0.08).  The P3 (0.32 ± 0.35) showed a significant reduction in eosinophil count compared with the P2 group. Neutrophil count decreased in the P3 group (6.5 ± 8.1) compared with the group K0 (10.9 ± 3.7) and P2 (13.5 ± 4.2). Lymphocyte count decreased in P2 (78.1 ± 7.3) compared to K0 (86.3 ± 3.9), and increases in P3 (87.4 ± 6.5) compared to P2. Monocytes in  P1, P2 and P3 showed no significant difference (2.0 ± 0.5).

                            Keywords: sleep deprivation, leukocytes, eosinophil, neutrophil, limfosit, monocyte

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Published

2016-09-01

How to Cite

Tri Novriansyah, W., Nasrul, E., & Rasyid, R. (2016). Efek Pengurangan Durasi Tidur terhadap Jumlah dan Hitung Jenis Leukosit pada Tikus Wistar. Jurnal Kesehatan Andalas, 5(3). https://doi.org/10.25077/jka.v5i3.607

Issue

Vol. 5 No. 3 (2016)

Section

Research Articles

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