Isolasi dan Karakterisasi Bakteri Endofit dari Tanaman Binahong dan Katepeng China
DOI:
https://doi.org/10.25077/jka.v3i2.33Abstract
Abstrak
Bakteri endofit merupakan mikroorganisme menguntungkan yang berinteraksi dengan tanaman inang tanpa menyebabkan gangguan atau kerusakan pada tanaman.Beberapa studi menunjukkan bahwa bakteri endofit tertentu dapat memproduksi senyawa kimia yang memiliki efek bagi kesehatan, terutama bakteri endofit yang diisolasi dari tanaman obat.Binahong (Anredera cordifolia) dan Ketepeng Cina (Cassia alata) merupakan tanaman obat yang telah diketahui memiliki beberapa khasiat.Tujuan dari penelitian ini ialah mengisolasi dan mengkarakterisasi bakteri endofit dari kedua tanaman tersebut melalui uji antibakteriterhadap tiga jenis bakteri patogen manusia (Pseudomonas aeruginosa, Staphylococcus aureus, dan Bacillus cereus). Metode: Jumlah bakteri endofit yang berhasil diisolasi dari dua tanaman tersebut berjumlah 73 isolat (37 isolat dari Binahong dan 36 isolat dari Ketepeng Cina). Hasil: Berdasarkan uji antibakteri terhadap tiga bakteri patogen diperoleh 16 isolat bakteri endofit yang memiliki aktivitas potensial (ditandai dengan terbentuknya zona hambat), yaitu 1 isolat dari Ketepeng Cina (terhadap Pseudomonas aeruginosa) dan 15 isolat dari Binahong. Pembahasan: Terbentuknya zona hambat mengindikasikan kemungkinan adanya senyawa kimia yang memiliki efek antibakteri.
Kata kunci: bakteri endofit, Anredera cordifolia, Cassia alata, antibakteri.
Abstract
Endophytic bacteria is a beneficial microorganism that interacts with plant without causing any harm to the host. Most studies showed that certain endophytic bacteria can produce chemical compound which have medical effect, especially endophytic bacteria isolated from medicinal plant. Binahong (Anrederacordifolia) and KetepengCina (Cassia alata) are several of medicinal plants potential to be used for medical purpose. Methods: The objective of this study was to isolate and to characterize endophytic bacteria from both plants through antibacterial activity test against to three kinds of human pathogenic bacteria (Pseudomonas aeruginosa, Staphylococcus aureus, and Bacillus cereus). Results: total isolated endophytic bacteria from both plants are 73 isolates (37 isolates from Binahong and 36 isolates from KetepengCina). There were 16 isolates have potential activity (indicated by clear zone), one isolate from KetepengCina and 15 isolates from Binahong. Discussion: The formed clear zone indicates antibacterial compounds produced by endophyticbacteriatested. Further research is needed to identify the type of antibacterial compounds produced.
Keywords:endophytic bacteria, Anrederacordifolia, Cassia alata, antibacterial.
Downloads
Published
How to Cite
Issue
Section
License
Authors retain copyright and grant the Jurnal Kesehatan Andalas right of first publication with the work simultaneously licensed under a Creative Commons Attribution-ShareAlike (CC BY-SA 4.0) license that allows others to share (copy and redistribute the material in any medium or format) and adapt (remix, transform, and build upon the material) the work for any purpose, even commercially with an acknowledgement of the work's authorship and initial publication in Jurnal Kesehatan Andalas.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in Jurnal Kesehatan Andalas.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
